FIG. 1.
BHV-5 genomic structure and schematic maps of gE recombinant plasmids. The genomic organization of BHV-5 depicted at the top consists of unique long (UL) and short (US) regions and two repeat regions (IR) and (TR). Localization of gE gene is indicated (A and B), and the region encompassing the gE gene (NotI/XhoI) is enlarged (C). A restriction site map of the NotI and XhoI fragment was generated, and various restriction endonuclease subfragments, indicated as bold lines, were cloned and sequenced. The arrow represents the BHV-5 gE ORF, and the arrowhead shows the direction of transcription. The regions of interest are shown in the schematic structures of plasmids pBHV-5gEΔβ (D), pBHV-5gE1 (E), and pBHV-1gE5 (F).