FIG. 1.

Effect of Rosco on HSV replication when added at 3-h intervals after infection. Vero cells were infected with 2.5 PFU of HSV-1 per cell. After 1 h of adsorption, the cells were washed and overlaid with medium containing no drug (control) or 100 μM Rosco. At 3, 6, 9, 12, 15, 18, and 21 h p.i., medium was removed and replaced with medium containing 100 μM Rosco (dotted lines, solid triangles). Changes from drug-free to Rosco-containing medium are indicated by the arrows. A second set of infected monolayers was left in drug-free medium (solid line, solid squares). At 1, 3, 6, 9, 12, 18, 21, and 24 h p.i., cultures were harvested and viral titers were determined by a standard plaque assay. Viral titers are plotted as a function of time p.i. Each time point indicates the average of two independent experiments.