FIG. 4.

Effect of Rosco and PAA on the steady-state levels of selected viral transcripts when drug is added at the time of release from a 12-h PAA block. Vero cells were infected with 2 PFU of HSV per cell, and medium was replaced as described in the legend to Fig. 3B. Immediately before release (0) and at 4, 8, and 16 h after release (hpr) from the PAA block and addition of the secondary drug, cells were harvested and total RNA was extracted. RNA extracted from mock-infected cells served as a negative control (MI). Levels of ICP0 (IE), ICP8 (E), and TK (E) transcripts were determined by RNase protection assays.