FIG. 6.

Replication of four HSV ts mutants after the shift-down from the nonpermissive to the permissive temperature in the presence of Rosco. Vero cells were infected at the nonpermissive temperature with 2.5 PFU of the indicated HSV ts mutants per cell. At 6 h p.i., infected cultures were transferred to the permissive temperature in the absence of drug (Control) or in the presence of 100 μM Rosco. One culture infected with each ts mutant was harvested immediately before the shift-down. At 24 h after the shift-down, the remaining infected monolayers were harvested and viral replication was monitored by a standard plaque assay. Fold viral replication after release was determined by dividing the titers at 24 h postrelease by the titers measured before release and subtracting 1.