FIG. 2.

The fate of VP22-induced microtubule bundles in transiently transfected cells. COS-1 cells grown on 42-mm-diameter coverslips were transfected with the expression vector for GFP-22 and transferred to a heated chamber 20 h later. A single cell containing bundled microtubules was selected (0 hrs), and images were collected every 10 min for a period of 24 h. Two-hourly images are shown in the gallery, and the corresponding animation can be found elsewhere (http://www.mcri.ac.uk/VirusAssembly/timelapse.html).