FIG. 3.

Translocation of GFP-22 from cytoplasm to nucleus during cell division. COS-1 cells grown on 42-mm-diameter coverslips were transfected with the expression vector for GFP-22 and transferred to a heated chamber 24 h later. A single cell containing diffuse cytoplasmic GFP-22, which had the appearance of a cell entering mitosis, was selected (0′), and images were collected every 2 min for a total time of 80 min. Representative images are shown in the gallery, and the corresponding animation can be found elsewhere (http://www.mcri.ac.uk/VirusAssembly/timelapse.html).