FIG. 7.

Comparison of the predicted amino acid sequences encoded by Sint1 cDNA and a human brain cDNA (KIAA0991; accession no., AB023208) or the human MSF cDNA (accession no., AF123052). The KIAA0991 and MSF sequences are 100% identical in the region shown. Boxed are the GTP-binding motifs. The sequences show 97.0% identity and 97.9% similarity (it should be noted, however, that the KIAA0991 and MSF sequences are 88 and 234 aa residues longer, respectively, than the Sint1 sequence). Alignment was performed with the Genetics Computer Group program BESTFIT. Vertical lines, identical residues; colons, well-conserved replacements that scored better than 0.5 in the PAM-250 matrix; dots, replacements scoring better than 0.1. The lengths of the Sint1, KIAA0991, and MSF sequences are 334, 422, and 568 aa, respectively.