TABLE 1.

Annotation of features used in PLS-DA models and their fold changes. Experimental m/z values are provided. Fold change (FC) was calculated as the ratio of injured/control (>1 means increase in injured). Mass-to-charge and mass error values were measured on a separate brain section. LC-MS/MS data was collected to confirm the lipid headgroups and to determine chain length. PC: Phosphocholine, ND: Not detected. HC: hippocampus, OC: outer cortex, WM: white matter, GM: gray matter, CC: corpus callosum.

m/z	Mass error (ppm)	ID	LC-MS/MS	FC HC	FC OC	FC WM	FC GM	FC CC	Model
480.3452	0.66	LPC(O-16:1) [M + H]+	PC	1.70	1.35	2.61	1.69	2.03	WM,GM, OC, CC
496.3399	0.33	LPC(16:0) [M + H]+	PC	1.27	1.20	1.28	1.23	1.29	All
506.3609	0.86	LPC(O-18:2) [M + H]+	PC	1.74	0.76	2.64	2.50	1.70	WM, OC
522.3559	0.83	LPC(18:1) [M + H]+	PC	1.49	1.43	1.22	1.45	1.20	GM, OC
732.5539	0.13	PC(32:1) [M + H]+	PC(16:0/16:1)	1.05	0.90	1.03	0.96	1.06	OC
782.5690	−0.50	PC(36:4) [M + H]+	PC(16:0/20:4)	1.01	0.99	1.03	1.00	1.03	WM
787.6688	0.00	SM(d40:1) [M + H]+	PC	0.45	1.48	1.47	1.18	2.10	WM
788.6162	−0.24	PC(36:1) [M + H]+	PC(18:0/18:1)	0.93	0.96	0.97	1.02	0.96	HC
806.5688	−0.81	PC(38:6) [M + H]+	PC(16:0/22:6)	0.97	1.06	0.99	1.05	1.00	OC
810.6009	0.16	PC(38:4) [M + H]+	PC(18:0/20:4)	0.97	0.95	0.99	0.97	0.97	OC
824.5564	−0.21	PC(36:2) [M + K]+	PC(18:1/18:1)	0.84	0.95	0.83	0.88	0.84	WM
826.5720	−0.36	PC(36:1) [M + K]+	PC(18:0/18:1)	0.83	0.92	0.88	0.89	0.92	WM, CC
834.6010	0.26	PC(40:6) [M + H]+	PC(18:0/22:6)	0.91	0.96	0.96	1.00	0.95	HC
838.6319	−0.22	PC(40:4) [M + H]+	PC(16:0/24:4)	0.94	0.84	1.01	0.95	1.06	OC
851.6403	0.04	SM(d42:2) [M + K] +	PC	0.45	1.17	0.99	1.03	1.07	HC
864.6329	0.42	HexCer(42:2;O3) [M + K] +	Not conclusive	0.70	ND	1.33	1.80	1.34	CC
866.6479	−0.29	HexCer(42:1;O3) [M + K] +	Not conclusive	0.88	0.62	1.21	1.73	1.31	GM, WM, CC