FIG. 3.

Southern blot for HTLV-1 integrated in B-cell genomic DNA generated from a patient with HAM/TSP (lanes 1 and 2), the SI-1 B cell line (lanes 3), and an HTLV-1-infected cell line from a patient with ATL (lanes 4). The line from the ATL patient contains a monoclonal integration of the HTLV-1 provirus. The other lines harbor multiple integration sites of the virus. Following digestion with PstI, expected virus-specific bands are 2.4, 1.6, and 1.3 kb; additional bands at higher molecular sizes are attributed to viral sequences contiguous with cellular genomic DNA external to adjacent PstI sites in the viral genome. Digestion of prototype virus with EcoRI predictably yields bands greater than 9 kb, due to a lack of internal EcoRI sites.