FIG. 4.

(a) Antigen capture assay for HTLV-I Gag. Hut102 and MT-1 are HTLV-1-infected T-cell lines; MT-1 is known to have very low levels of viral transcriptional activity. UN-1 is a B-cell line generated from an HTLV-1-seronegative individual. Black and grey columns represent cell supernatant and cell lysate fractions, respectively. (b) Western blot assay for HTLV-1 p19. Western blot analysis was performed on supernatants (Spt) and cell lysates (Ly) from an uninfected B-cell line (UN-1), a B-cell line derived from seroindeterminate patient SI-1 (SI-1 B), a B-cell line from a HAM/TSP patient (HAM/TSP-1), and an HTLV-1 infected T-cell line (Hut102) with a monoclonal antibody to HTLV-1 p19 at a 1:50 dilution. SeeBlue (Novex, San Diego, Calif.) markers (not shown) were used for gel calibration. (c) RT-PCR assay for HTLV-1 tax, pol, and env. UN-1 is an HTLV-1-uninfected B-cell line; Hut102 is an HTLV-1-infected T-cell line; Jurkat is an HTLV-1-uninfected T-cell line; HAM/TSP is an HTLV-1-infected B-cell line from a HAM/TSP patient: SI-1 is a B-cell line derived from seroindeterminate patient SI-1.