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. 2024 Jun 1;104:105170. doi: 10.1016/j.ebiom.2024.105170

Fig. 3.

Fig. 3

Therapeutic effects of anti-EBOV-GP antibodies on acute retinal inflammation in VSV-EBOV-infected mice. P3 C57BL/6 mice were infected, treated as above at 3 dpi, and sacrificed at 9 dpi. (a) Histopathology of the eyes of uninfected mice (n = 4) and VSV-EBOV-infected treated with IgG control (n = 10), polyAb (n = 7), ADCC-mAb (n = 6), NEUT-mAb (n = 4) or the combination of ADCC-mAb and NEUT-mAb (n = 5). Left images show representative H&E staining of eyes. Right graph shows the retinal inflammation score. Data are shown as mean + SD. (b) Left images show representative immunofluorescence staining for TUNEL (green) and DAPI (blue) in eye sections from uninfected mice (n = 5) and VSV-EBOV-infected mice treated with IgG control (n = 6), polyAb (n = 3), ADCC-mAb (n = 6), NEUT-mAb (n = 4) or the combination of ADCC-mAb and NEUT-mAb (n = 5). Scale bar: 50 μm. Images of the full eye can be found in Supplementary Figure 6. Right graph shows the quantification of TUNEL + cells per field of view (3 fields per eye section). Data are shown as mean + S.D. (n = 9–18 fields for each group). (c) The images show representative immunofluorescence staining for CD45 (green), IBA (red) and DAPI (blue) in eye sections from uninfected mice (n = 5) and VSV-EBOV-infected mice treated with IgG control (n = 6), polyAb (n = 3), ADCC-mAb (n = 6), NEUT-mAb (n = 4) or the combination of ADCC-mAb and NEUT-mAb (n = 5). The bottom close-up images show representative microglia morphology observed in eyes. Scale bar: 50 μm. Images of the full eye can be found in Supplementary Figure S6. (d) Flow cytometry analysis of cells isolated from the eyes collected at 9 dpi. The graphs show the percentage of infiltrating immune cells (CD45+) and T cells (CD45+CD3+NK1.1−) in the eyes from uninfected mice (n = 3) and VSV-EBOV-infected mice treated with IgG control (n = 5), polyAb (n = 4), ADCC-mAb (n = 7), NEUT-mAb (n = 5) or the combination of ADCC-mAb and NEUT-mAb (n = 5). Data are shown as mean + SD. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 compared to uninfected mice. #P < 0.05 and ##P < 0.01 compared to VSV-EBOV-infected mice treated with IgG control.