Fig. 2. Body temperatures, nasal viral shedding and viremia in old and young horses after intranasal infection with EHV-1. (a) Mean body temperatures (°F). The dotted horizontal line represents the fever cut off at 101.5 °F. (b) Viral nasal shedding is expressed as log₁₀EHV-1 gB copy number per 100 ng DNA detected in nasal swabs by qPCR. (c) viremia is shown as log₁₀EHV-1 gB copy number per 500 ng DNA in PBMCs evaluated by qPCR. The data are presented as mean values (±sem). *Significant differences of viral load among groups (nasal viral shedding, viremia) or body temperature (P<0.05).