Table 1. Experimental design.
Day of study | −4 | −3 | CH | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 21 | |
Physical exams | x | x | x | x | x | x | x | x | x | x | x | x | x | x | x | x | x | ad | x | ||
Body temperatures | x | x | x | x | x | x | x | x | x | x | x | x | x | x | x | x | ad | x | |||
Shedding | x | x | x | x | x | x | x | x | x | x | x | x | x | x | x | x | x | ad | x | ||
Viremia | x | x | x | x | x | x | x | x | x | x | x | ||||||||||
EHV-1 VN and IgG Ab titres | x | x | x | ||||||||||||||||||
Cytokines in nasal secretions | x | x | x | ||||||||||||||||||
Cytokines/chemokines in mRNA | x | x | x | x | x | x | x | x | x | x | x | ||||||||||
Cytokines in CSF | x | x |
ad, Sampling on alternate days; x, daily sampling; CH, challenge infection.