Biochemical analysis of skeletal muscle in mstn-deficient red sea bream. (a) Drip loss (DL), (b) pH, and (c) lactic acid content in fish fillets stored in refrigeration. The first measurement was conducted at 9 h postmortem, followed by periodic evaluations every 24 h until the fourth day postmortem. The group of wild type and homozygous mstn mutants are designated as “WT” and “HM”, respectively. The genotype has a significant impact on the DL, pH, and lactic acid content according to the two-way ANOVA test (P = 0.000837, 0.000292, and 0.00000644, respectively). The asterisks indicate that the values are significantly different by Sidak's test, *: P < 0 0.05. The daggers indicate that the values tend to be different by Sidak's test, †: P < 0.1. Data are presented as the mean ± SD (n = 5). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)