Quantification of collagen content in mstn-deficient red sea bream. A total of three measurements were performed at 18, 42, and 90 h postmortem, which are labeled “Day 0”, “Day 1”, and “Day 3”, respectively. The group of wild type and homozygous mstn mutants are designated as “WT” and “HM”, respectively. The genotype has a significant impact on the collagen content, according to the two-way ANOVA test (P = 0.000539). The daggers indicate that the values tend to be different by Sidak's test, †: P < 0.1. Data are presented as the mean ± SD (n = 5). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)