FIG. 5.

Footprinting analysis reveals that 3CD induces dissociation of PCBP from stem-loop IV. The footprinting analysis was performed as described for Fig. 1A. The viral 5′UTR was treated with RNase T₁ (lanes 1 to 12) or RNase T₂ (lanes 13 to 24). RNase was added in the presence of bovine serum albumin (−), 3CD, PCBP, or 3CD plus PCBP proteins, as indicated on the top of each lane. The numbers on the left indicate the nucleotide position in the poliovirus type 1 genome.