Skip to main content
. 2024 May 25;96(23):9643–9652. doi: 10.1021/acs.analchem.4c01388

Figure 2.

Figure 2

Development of a comprehensive LC method for signaling lipid analysis. (A) Extracted ion chromatograms (XICs) of free phosphate group-containing analytes LPA, S1P, and CerP. Three different columns only differing in the type of column hardware were tested using 0.1% HAc as an eluent additive. “SS” (stainless steel) corresponds to YMC-Triart, “PEEK” to YMC-Triart metal-free, and “coated” to YMC-Accura Triart. (B) Relative signal intensities for the three tested columns (n = 4). (C) Carryover of free phosphate group-containing analytes in dependency of the column coating. Carryover was calculated as the ratio between the peak area of the blank injection after the standard mix injection and the peak area of the standard mix injection itself and is expressed as a percentage. (D) Signal intensities for different salt and acid concentrations using the YMC-Accura Triart column (n = 4). (E) Carryover of LPA, S1P, and CerP using the YMC-Accura Triart column and different salt and acid concentrations. (F) Example of improved peak shape of LPA 17:1 after final method optimization.