FIG. 7.
High doses of IFNα administered systemically inhibit HBV replication. Age-, sex-, and serum HBeAg-matched transgenic mice (lineage 1.3.46) were injected intravenously with various doses of IFNα, and their livers were harvested 24 h after injection for Southern analysis of HBV DNA replicative forms and Northern analysis of 2′,5′-OAS RNA exactly as described in the legend to Fig. 1. Results were compared with those for livers pooled from 10 age-, sex-, and serum HBeAg-matched transgenic saline-injected controls (NaCl). Results were also compared with those for livers harvested 24 h after injection with poly(I · C) (200 μg/mouse) or infection with LCMV (2 × 106 PFU/mouse) or adenovirus (1.5 × 109 PFU/mouse). RC and SS, relaxed-circular and single-stranded HBV DNA replicative forms, respectively.