FIG. 1.

Levels of CAT activity from the ORF4 promoter in the presence of IE62 and in the presence or absence of the protein kinases from VZV ORF47 (+47) or VZV ORF66 (+66). Levels were derived from duplicate transfections on 35-mm-diameter well dishes. Transfections contained 1 μg of the ORF4 promoter CAT construct, 1 μg of pCMV-gal, 200 ng of pKCMV62, and 1 μg (total) of a mix of pG310 and either pKCMV66 or pKCMV47. The x axis indicates the amount of plasmid pKCMV47 or pKCMV66 used in the cotransfection. CAT activity at 0 μg represents a 36-fold increase over CAT activity obtained from transfections lacking pKCMV62.