Immunofluorescent images showing nuclear localization of IE62 peptides and modulation by ORF66 protein kinase. The IE62 peptide shown in panels A and B is expressed from pKCMV62d730-T, panels C and D represent protein expressed from pKCMV62d161-505, panels E and F are from pKCMV62d29-602, and panels G and H represent the IE62 HA-tagged peptide from 506-734, expressed from plasmid pGK2HA5. Panels A, C, E, and G represent the protein expressed alone, whereas panels B, D, F, and H represent the protein expressed following transfection with a threefold excess of pKCMV66.