FIG. 4.

Plaques produced by wild-type HSV-1 and gE mutants. HEC-1A, ARPE-19, HaCaT, or Camcell-1 cells were infected with wild-type HSV-1 (strain F), F-gEβ, F-gEΔCT, F-gESer, or F-gE-US7/8 using 0.001 to 0.0001 PFU/cell. Two hours later the virus inoculum was removed and cells were overlaid with media containing 0.3% human gamma globulin, a source of anti-HSV-neutralizing antibodies. After 2 days the cells were fixed and stained for HSV antigens. (A) Photomicrograph of plaques formed on HEC-1A, ARPE-19, and HaCaT cells. (B) Twenty plaques produced by each virus were photographed and subjected to image analysis to quantify the area of the plaque.