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. 2024 May 31;14(11):1657. doi: 10.3390/ani14111657

Table 2.

Effects of dietary different levels of COS on the rumen protozoa count of beef cattle.

Items Dietary COS Level, Feeding Basis SEM p-Value
CON 0.02%
COS
0.04%
COS
0.08%
COS
Number of Rumen Protozoa on Days 1–8 (105/mL)
Day 1 A 7.86 7.86 7.86 7.86 0 /
Day 2 2.82 2.84 2.85 2.73 0.072 0.374
Day 3 1.13 1.22 1.21 1.15 0.057 0.357
Day 4 0.74 0.73 0.74 0.72 0.023 0.783
Day 5 0.67 0.68 0.68 0.64 0.029 0.441
Day 6 0.57 a 0.58 a 0.54 ab 0.50 b 0.024 0.015
Day 7 0.49 ab 0.51 a 0.46 ab 0.44 b 0.028 0.065
Day 8 0.47 a 0.46 ab 0.44 ab 0.42 b 0.020 0.144

In the table, the superscript “a–b” represents the differences in data between the treatment groups. Different letters indicate differences between the groups (p < 0.05), while the same or no letters indicate no significant differences (p > 0.05); SEM, standard error of the mean. A On the first day, all fermentation tanks were filled with a solution of fermentation broth and RUSITEC system buffer for rumen protozoa counting. This solution was evenly distributed throughout the tanks, ensuring that the protozoa count on the first day was consistent.