Table 1.
Variant ID | Primer Names | Primer Sequences (5′ to 3′) | Tm (°C) | Product Sizes (bp) |
---|---|---|---|---|
INS.57332 | P1 | F: TTATGAGCTTCTCTCCGCCAG | 59.59 | 514/682 |
R: CTTCAGCGAGCTTCGAGACA | 60.11 | |||
P2 | F: CTGGGTTGTTCCCAACTGGA | 59.82 | 510/678 | |
R: AGCCTTCAATCTCCTTGGCG | 60.39 | |||
P3 | F: CCGACGTAGCTGGGTTGTTC | 61.01 | 488/656 | |
R: GGTATAATTGCCGGGCTCCAT | 60.27 |