FIG. 5.

At a late stage of RRV infection, [Ca²⁺]_i increase in Caco-2 cells partially depends on a PLC mechanism. U-73122, a PLC inhibitor, or U-73343, a close analog, was added to Caco-2 cells at time zero. Mock-infected and RRV-infected cells were trypsinized at indicated times p.i. to measure [Ca²⁺]_i by quin2 fluorescence. Values are means ± standard deviations from at least six experiments. Statistical differences between control and U-73122- or U-73343-treated cells were determined by Student's t test. NS, not significantly different; ∗, P < 0.01.