Table 1.
Locus | Primer Sequences | Amplicon | Tm(°C)* |
---|---|---|---|
PGR | For: ATCCTACAAACACGTCAGTGGGCA Rev: ACTGGGTTTGACTTCGTAGCCCTT |
139bp | 60.5 60.3 |
PDGFR | For: GACTTTCGCCAAAGTGGAGGAG Rev: AGCCACCGTGAGTTCAGAACGC |
121bp | 58.0 62.0 |
FOS | For: GCCTCTCTTACTACCACTCACC Rev: AGATGGCAGTGACCGTGGGAAT |
123bp | 56.5 60.9 |
JUN | For: CGATCTGCACAAGATGAACCACG Rev: CTGCTGAGGTTGGTGTAAACGG |
106bp | 58.3 58.2 |
STAT3 | For: CTTTGAGACCGAGGTGTATCACC Rev: GGTCAGCATGTTGTACCACAGG |
133bp | 57.1 58.1 |
18S rRNA | For: AACTTTCGATGGTAGTCGCCG Rev: CCTTGGATGTGGTAGCCGTTT |
104bp | 57.3 57.6 |
Tm is estimated in the presence of 1.5mM MgCl2 using OligoAnalyzer software available on-line at Integrated DNA Technologies (www.idtdna.com).