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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2023 Sep 15;234:106399. doi: 10.1016/j.jsbmb.2023.106399

Table 1.

Primers used for quantitative PCR (qPCR).

Locus Primer Sequences Amplicon Tm(°C)*
PGR For: ATCCTACAAACACGTCAGTGGGCA
Rev: ACTGGGTTTGACTTCGTAGCCCTT
139bp 60.5
60.3
PDGFR For: GACTTTCGCCAAAGTGGAGGAG
Rev: AGCCACCGTGAGTTCAGAACGC
121bp 58.0
62.0
FOS For: GCCTCTCTTACTACCACTCACC
Rev: AGATGGCAGTGACCGTGGGAAT
123bp 56.5
60.9
JUN For: CGATCTGCACAAGATGAACCACG
Rev: CTGCTGAGGTTGGTGTAAACGG
106bp 58.3
58.2
STAT3 For: CTTTGAGACCGAGGTGTATCACC
Rev: GGTCAGCATGTTGTACCACAGG
133bp 57.1
58.1
18S rRNA For: AACTTTCGATGGTAGTCGCCG
Rev: CCTTGGATGTGGTAGCCGTTT
104bp 57.3
57.6
*

Tm is estimated in the presence of 1.5mM MgCl2 using OligoAnalyzer software available on-line at Integrated DNA Technologies (www.idtdna.com).