Figure 1.
Cell viability percentage measured by MTT assay. RSpN cells (0.75 × 105 cells/mL) were exposed to TeNT and mAbs. The TeNT was pre-incubated with antitetanus mAbs or isotype control for 1 h at 37 °C (1:2 molar ratio, 25 mM). The experimental conditions comprised control (cells only in the black bar), TeNT in the gray bar, isotype control in the white bar, TeNT plus isotype control in the orange bar, TT-117 mAb in the pink bar, and TT-140 in the blue bar. The percentages are the average of two independent experiments in quadruplicate each.