Figure 4.

Design and recombinant expression of full-length VsAly7A and its truncated mutants. (A) Domain structure of full-length VsAly7A and its truncated mutants. (B) SDS-PAGE illustrating the full-length and other soluble truncated mutants. Lane M, molecular weight markers. Lane 1, VsAly7A-FL. Lane 2, PDT-CD I. Lane 3, PDT-CD II. Lane 4, CD I-CD II. (C) SDS-PAGE displaying FL VsAly7A and its truncated mutants under optimal induction conditions. Lane M, molecular weight markers. Lane a, induced cell lysate of E. coli cells. Lane b, supernatant fluid of the induced cell lysate. Lane c, precipitate of the induced cell lysate.