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. 2024 May 26;25(11):5801. doi: 10.3390/ijms25115801

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Substrate specificities and degradation of VsAly7A and its four soluble truncated mutants. (A) Substrate specificities of VsAly7A and its truncated mutants. (B) The time course of alginate degradation by FL VsAly7A and its truncated mutants were determined using TLC. (C,D) Analysis of end products of VsAly7A and PDT-CD I. (C) FPLC chromatogram. (D) ESI-MS analysis. The elution volumes of 16.04, 15.01, 14.16, 13,65, and 12.97 mL corresponded to unsaturated dis-, tri-, tetra-, penta- and hexasaccharide, respectively.

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