Figure 2.

R. roxburghii fruit extract decreases reactive oxygen species levels, enhances cellular superoxide dismutase activity, and alleviates oxidative stress in mesenchymal stem cells. (A,B) Reactive oxygen species were measured with a Beyotime ROS Assay Kit using a DCFH-DA probe to analyze ROS levels by FACS analysis (A) after treatment with R. roxburghii fruit extract (CL-3) or vitamin C (Vc) for 48 h. The results of the quantitative analysis of (A) are shown in (B). PBS was used as a negative control (NC). (C) SOD activity was detected by a superoxide dismutase assay kit with NBT after R. roxburghii fruit extract treatment for 48 h. (D,E) Mesenchymal stem cells were pretreated with R. roxburghii fruit extract or vitamin C for 48 h and treated with 200 μΜ H₂O₂ for 4 h (D). Scal bar = 100 μm. The relative ROS level was detected by FACS analysis (E). Student’s t-test, * p < 0.05, ** p < 0.01, **** p < 0.0001.