FIG. 6.

CAT expression analysis. Viral preparation of the pCC, pICUT-C, and pICUT-2–C vectors schematically described in the figure were produced by transient transfection. This was then used to transduce 293T cells (with a multiplicity of infection of 1; calculated from established titers of equivalent vectors with a marker gene in place of CAT), and the CAT activity from cell lysates was subsequently assayed. The average fold increase in CAT activity observed by the two pICUT vectors over that of the pCC control vector is shown.