Figure 4.

Proteomic analysis of soluble seed proteins from CRISPR-Cas9 BBi mutant and control lines. (A) Sodium dodecyl sulfate polyacrylamide gel electrophoresis of calcium fractionated soluble seed proteins visualized with Coomassie Blue; (B) immunoblot using Bowman–Birk inhibitor (BBi)-specific antibodies; (C) immunoblot using KTi-specific antibodies (KTis). Samples in order are ‘Maverick’ (Mav., non-transgenic control); 2 = Event 2; 3 = Event 3; 4 = Event 4; 5 = Event 5; 7 = Event 7; 8 = Event 8.