Table 1.
Analysis of trypsin inhibitor activity and chymotrypsin inhibitor activities in seeds from transgenic CRISPR-Cas9BBi-mutagenized and control plants. Tukey’s HSD indicates the results of a Tukey–Shapiro Honest Square Difference test; presence of the same letter indicates insignificant differences between genotypes.
| Seed Powder | Seed Protein | ||||
|---|---|---|---|---|---|
| Genotype | n= | Mean ± SD CIU/mg | Tukey’s HSD | Mean ± SD CIU/mg | Tukey’s HSD |
| ‘Maverick’ (WT) | 3 | 17.2 ± 1.91 | A | 56.93 ± 6.32 | A |
| KTi-3 | 3 | 18.55 ± 0.42 | A | 60.97 ± 1.39 | A |
| Event 5 | 3 | 15.93 ± 1.66 | A | 56.45 ± 5.89 | A |
| Event 3 | 3 | 15.35 ± 1.63 | A | 51.34 ± 5.44 | A |
| Event 4 | 3 | 6.2 ± 1.77 | B | 21.15 ± 6.03 | B |
| Event 2 | 3 | 4.19 ± 1.03 | B | 15.28 ± 3.77 | B |
| Event 7 | 3 | 3.9 ± 1.24 | B | 16.85 ± 5.38 | B |
| Event 8 | 3 | 2.89 ± 0.04 | B | 11.54 ± 0.17 | B |
| Seed Powder | Seed Protein | ||||
| Genotype | n= | Mean ± SD TIU/mg | Tukey’s HSD | Mean ± SD TIU/mg | Tukey’s HSD |
| ‘Maverick’ (WT) | 3 | 57.25 ± 0.35 | A | 189.48 ± 1.17 | A |
| KTi-3 | 3 | 36.45 ± 1.63 | B | 119.8 ± 5.35 | BC |
| Event 5 | 3 | 35.7 ± 1.27 | B | 126.55 ± 4.51 | B |
| Event 3 | 3 | 31.96 ± 1 | C | 106.88 ± 3.35 | C |
| Event 4 | 3 | 24 ± 0.59 | D | 81.87 ± 2.01 | D |
| Event 2 | 3 | 21.38 ± 0.06 | DE | 77.97 ± 0.21 | D |
| Event 7 | 3 | 19.75 ± 1.89 | E | 85.35 ± 8.15 | D |
| Event 8 | 3 | 14.46 ± 1.71 | F | 57.74 ± 6.82 | E |