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. 2024 May 24;25(11):5735. doi: 10.3390/ijms25115735

Figure 1.

Figure 1

Aortic Ca content and gene expression from control (SHAM) and nephrectomized (NX) rats with and without parathyroidectomy, fed normal (NP) or high (HP) phosphorus diet for 18 weeks. Aortic Ca content was determined by o-cresolphtalein complexone method (A). Gene expression was evaluated by qRT-PCR of: Lgr4 (B), Rankl (C), Opg (D), Rspo1 (E), Rspo2 (F), Rspo3 (G) and Rspo4 (H). The groups are SHAM NP (Sham-operated rats fed normal phosphorus diet), SHAM HP (Sham-operated rats fed high phosphorus diet), PTX NX NP (parathyroidectomized and nephrectomized rats fed normal phosphorus diet), PTX NX HP (parathyroidectomized and nephrectomized rats fed high phosphorus diet), NX NP (nephrectomized rats fed normal phosphorus diet), and NX HP (nephrectomized rats fed high phosphorus diet). Data are presented as median [interquartile range]. R.U., relative units. a p < 0.05, aa p < 0.01, aaa p < 0.001 versus SHAM NP; b p < 0.05, bb p < 0.01, versus SHAM HP; c p < 0.05 versus PTX NX NP; dd p < 0.01 versus PTX NX HP; ee p < 0.01 versus NX NP.