Figure 2.
Effect of high phosphate (Pi) (3 mM) and different PTH concentrations (10−9, 10−8, and 10−7 M) on A7r5 vascular smooth muscle cells after 4 days of exposure. Ca content was determined by the o-cresolphtalein complexone method (A). Gene expression was evaluated by qRT-PCR for Lgr4 (B), Rankl (C), Opg (D), Rspo1 (E), Rspo2 (F), Rspo3 (G) and Rspo4 (H). Data are presented as median [interquartile range]. R.U., relative units. a p < 0.05, aa p < 0.01, aaa p < 0.001 versus control (non-calcifying medium, 1 mM phosphate); b p < 0.05 versus Pi.