Figure 4.

Effect of Lgr4 silencing on A7r5 vascular smooth muscle cells exposed to soluble RANKL (10 pM) in non-calcifying medium (1 mM phosphate). Ca content was determined by the o-cresolphtalein complexone method (A). Gene expression was evaluated by qRT-PCR for Alp (B), Runx2 (C), and α-actin (D). White and grey boxes represent the non-addition of RANKL and the addition of 10 pM of RANKL, respectively. Data are presented as median [interquartile range]. R.U., relative units. Mock, scramble transfection control of silencing. ^a p < 0.05, ^aa p < 0.01, ^aaa p < 0.001 versus Mock (0 pM RANKL); ^b p < 0.05, ^bb p < 0.01, ^bbb p < 0.001 versus Mock + 10 pM RANKL.