FIG. 5.

Genome resolution of viral DNA. BSC40 cells were infected with wt or tsH5-4 virus at an MOI of 2 and maintained at the indicated temperature. As controls, cells were infected with vR0G8 in the absence of IPTG or infected with wt virus in the presence of 60 μM IBT throughout infection. At 18 h p.i., cells were harvested and cytoplasmic DNA was prepared and digested with BstEII. Digested DNA was resolved on a 1% TAE gel, transferred to a ZetaProbe membrane, and hybridized with a radiolabeled probe recognizing the termini of the viral genome. The arrows indicate the fragments released from resolved mature genomes (1.3 kb) and unresolved concatamer genomes (2.6 kb). The sizes of DNA standards are indicated in kilobase pairs on the left.