Figure 5.

(A) Cartoon illustrating the structure of the fluorescent supramolecular construct ConA-Alexa647-strep. Biotin-(green)-labeled ConA tetramers (blue) bind Alexa647-(red)-labeled streptavidin tetramers (light orange). The interaction between ConA and the sugars on the surface of the bacterial wall drives the construct to the cellular target, in this example, S. aureus. Panels (B,C) Colocalization of bacterial DNA (green, widefield) and the ConA-Alexa647-strep construct (red, dSTORM) for S. aureus (B) and E. coli (C). For Syto13, excitation was at 488 nm; detection in the green channel was at 498–551 nm. For Alexa647, excitation was at 640 nm; detection in the red channel was at 685/40 nm.