Figure 8.

(a) N2a cells containing formazan crystals following staining with MTT in different experimental conditions: untreated (Control); treated with 5 µM Fer-Ger (Fer-Ger 5 µM); treated with 100 µM H₂O₂ (H₂O₂ 100 µM); treated with 100 µM H₂O₂ in the presence of 1 µM Fer-Ger (H₂O₂ + Fer-Ger 1 µM); treated with 100 µM H₂O₂ in the presence of 5 µM Fer-Ger (H₂O₂ + Fer-Ger 5 µM). (b) Effect of Fer-Ger on H₂O₂ induced oxidative stress in N2a cell viability. N2a cells were exposed to 1 and 5 µM Fer-Ger for 1 h followed by a treatment with 100 μM H₂O₂ for 24 h. Cell viability was measured by the MTT assay. Data are expressed as mean ± S.E.M. (n = 4). **** p < 0.0001 H₂O₂-treated cells compared to control (untreated cells) and to 1 and 5 µM Fer-Ger-exposed cells; statistical analyses were performed by one-way ANOVA, followed by Dunnett’s multiple comparisons test.