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. 2024 Jun 1;25(11):6100. doi: 10.3390/ijms25116100

Figure 4.

Figure 4

Effect of MIPP and MOMIPP on caspase-3/7 activity and cell viability in HT-1080 cells. HT-1080 cells were incubated for 6 h (A,C) and 24 h (B,D) in the presence of MIPP or MOMIPP (1 μM) with or without Z-VAD(OMe)-FMK (10 μM). The caspase-3/7 activity was determined by Apo-ONE® Homogeneous Caspase-3/7 Assay, and cell viability was determined by MTS assay. Each point (mean ± SEM of two independent experiments, each of which consisted of eight replicates per treatment group) represents the relative fluorescence units (RFU) and is expressed as a percentage of NT (nontreated cells) set as 100%. The statistical analysis by one-way ANOVA showed significant differences between the groups (α = 0.05) and was followed by Sidak’s multiple comparison test. Data indicated with *** p ≤ 0.001 (versus NT) and ### p ≤ 0.001 (versus Z-VAD treated cells) reflect statistically significant differences.