Figure 12.

Double immunolabeling for LC3b (in green) and lysosomes (in red) in controls (a–c) and differently treated U251 cells, i.e., after 48hr-CT using Ganostile 5 mg/mL (d–f), Cisplatin 40 μM (g–i), Pt(IV)Ac-POA 10 μM (j–l), Cisplatin + Ganostile (m–o), or Pt(IV)Ac-POA + Ganostile (p–r). DNA was stained with Hoechst 33258 (blue fluorescence). Magnification 60×; scale bar: 25 μm. (Panel A) Histogram showing the LC3b mean fluorescence intensity per cell (OD) in control and differently treated cells. * control vs. each experimental condition; ° Ganostile vs. other treatments; # Cisplatin vs. any combined treatment; ^ Pt(IV)Ac-POA vs. each combined treatment; + Cisplatin + Ganostile vs. other experimental groups.