Figure 6.

Antioxidant defence in the tumour microenvironment. (A,I,K) Enzyme activities were evaluated using a spectrophotometric assay measuring the disappearance or apparition of the co-substrate NADPH, H⁺. (D) Superoxide dismutase (SOD) activity was measured using a kit from Sigma-Aldrich. (G) Reduced glutathione (GSH) was quantified using a spectrophotometric assay measuring the appearance of the 2-nitro-5-thiobenzoate (TNB) after the reaction between GSH and 5,5′-dithiobis-TNB. (B,C,E,F,H,J,L) mRNA expression was determined by RT-qPCR and normalised with Hprt. Enzyme activity assays were performed on 4 mice/group and targeted transcriptomic analysis on 5 mice/group. Results are presented as boxes with median, interquartile range and min–max values. Data were analysed by a Mann–Whitney test, * p < 0.05, ** p < 0.01. ST and LT HFD: short- or long-term high-fat diet.