Table 3.
Antibodies used for flow cytometry staining.
| Cell Type | Antibody | Fluorochrome | Clone | Provider |
|---|---|---|---|---|
| TAM | Cd11b | PerCP Cy5.5 | M1/70.15 | eBioscience |
| F4/80 | PE | REA126 | MACS Miltenyi | |
| Natural Killer | NK1.1 | PerCP Vio®700 | PK136 | MACS Miltenyi |
| CD49b (DX5) | PE | DX5 | MACS Miltenyi | |
| T4 | CD4 | PE | GK1.5 | MACS Miltenyi |
| T8 | CD8α | FITC | 53-6.7 | MACS Miltenyi |
| Th1 | CD4 | AF700 | GK1.5 | eBioscience |
| CD119 | PE | REA189 | MACS Miltenyi | |
| Th2 | CD4 | AF700 | GK1.5 | eBioscience |
| CD124 | PE | REA235 | MACS Miltenyi | |
| Treg | CD25 | PE | PC61.5 | eBioscience |
| FoxP3 | Biotin | FJK-16s | eBioscience |
TAM: Tumour-associated macrophage, NK: natural killer, T4: T4 lymphocyte, T8: T8 lymphocyte, Th1: T helper 1 lymphocyte, Th2: helper 2 T lymphocyte, Treg; regulatory T lymphocyte, CD: cluster of differentiation, FoxP3: forkhead box protein 3; PerCP: peridinin chlorophyll protein, Cy: cyanine, PE: phycoerythrine, FITC: fluorescein isothiocyanate, AF700: Alexa Fluor 700.