Table 4.
PCR Primers.
| Gene | Species | Sequence Reference | Amplicon Size | Forward Primer | Reverse Primer |
|---|---|---|---|---|---|
| Hprt | mouse | NM_013556.2 | 176 | GTAATGATCAGTCAACGGGGGAC | CCAGCAAGCTTGCAACCTTAACCA |
| Cd45 | mouse | NM_001111316.2 | 158 | GCTGATGGATGTGGAGCCAA | TCTGATTGTGGGGCTTTCGG |
| Cpt1 | mouse | NM_013495.2 | 336 | CTGGCTCTACCATGACGGGA | ATGGACTTGTCAAACCACCTGTC |
| Cpt2 | mouse | NM_009949 | 241 | CATCGTACCCACCATGCACT | ATCAAACCAGGGGCCTGAGA |
| Cs | mouse | NM_026444.4 | 133 | CAGCAGTATCGGAGCCATTGAC | ACCACCCTCATGGTCACTATGGAT |
| Gclc | mouse | NM_010295.2 | 375 | TCGACCTGACCATCGATAAGGA | TCATGTTCTCGTCAACCTTGG |
| Gpx 1 | mouse | NM_008160.6 | 150 | GCTCATTGAGAATGTCGCGT | TCATTCTTGCCATTCTCCTGGT |
| Gpx 2 | mouse | NM_030677.2 | 108 | CGGGACTACAACCAGCTCAA | CTCGTTCTGACAGTTCTCCTGA |
| Gsr | mouse | NM_010344.4 | 177 | GGCACTTGCGTGAATGTTGG | ATAGATGGTGTTCAGGCGGC |
| Gsto1 | mouse | NM_010362.3 | 168 | CTCCGAACCTAAGGGAAGCG | TGTGGGCTAGACACTCCTTG |
| Sod1 | mouse | NM_011434.1 | 136 | GGAACCATCCACTTCGAGCA | CTGCACTGGTACAGCCTTGT |
| Sod2 | mouse | NM_013671.3 | 179 | GAACAATCTCAACGCCACCG | CCAGCAACTCTCCTTTGGGTT |
| Nos2 | mouse | NM_010927.4 | 131 | AGGGTCACAACTTTACAGGGAG | GTGAGGAGCCTCAGAAGTGTC |
| Mrc1 | mouse | NM_008625.2 | 102 | TTGCACTTTGAGGGAAGCGA | CCTTGCCTGATGCCAGGTTA |
Hprt: hypoxanthine-guanine phosphoribosyltransferase, Cd: cluster of differentiation, Cpt: carnitine palmitoyltransferase, Cs: citrate synthase, Gclc: glutamate-cysteine ligase catalytic subunit, Gpx: glutathione peroxidase, Gsr: glutathione-disulfide reductase, Gsto: glutathine S-transferase omega, Sod: superoxide dismutase, Nos2: nitric oxide synthase 2, Mrc1: mannose receptor C-type 1.