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Inhibitory effects of WT and mutant AAV genomes on activity of the HPV-16 LCR. (A) Plasmid constructs containing the WT or mutated AAV genome. ITR, inverted terminal repeat. (B) SiHa cells were cotransfected with 10 μg of reporter pBL-16LCR-CAT6 and either 5 μg of vector pBR322 or 10 μg of an effector plasmid (pAV1, pΔAAV1, or pΔKAV1) to yield a molar ratio of 2:1. The data are the means ± standard errors of four individual experiments.
