TABLE 1.

Sequences of the primers used to generate deletion mutant fragments of the HPV-16 LCR^a

End and fragment	Primer	Sequenceb
5′ end
F1	H16	CCCAAGCTTGTATAAAACTAAGGGCGTAA
F2	H7855	CCCAAGCTTAAACCGATTTTGGGTTACAC
F3	H7755	CCCAAGCTTAAACTTCTAAGGCCAACTAA
F4	H7729	CCCAAGCTTCCTAATTGCATATTTGGCAT
F5	H7661	CCCAAGCTTTAAATCACTATGCGCCCACG
F6	H7629	CCCAAGCTTCTGAATCACTATGTACATTG
F7	H7524	CCCAAGCTTAACTTGTACGTTTCCTGCTT
3′ end	100B	AATACGTGGTTTTCTCTTGACCTAGGCGC

^aEach primer at the 5′ end was individually coupled with the 3′-end primer to generate LCR fragments F1 to F7 (Fig. 3A). The PCR was performed per the manufacturer's instructions (GeneAmp PCR system 2400; Perkin-Elmer, Branchburg, N.J.). To clone the PCR-amplified fragments into the vector pBLCAT6 at the HindIII and BamHI sites, primers at the 5′ end and 3′ end containing a HindIII and BamHI restriction sites, respectively, were generated. The numbers after H (for HindIII site) in 5′-end primers and before B (for BamHI site) in the 3′-end primer indicate the nucleotide numbers in the HPV-16 sequence. 

^bAll 5′-end sequences are in the 5′-to-3′ direction, and the 3′-end sequence is in the 3′-to-5′ direction.