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. Author manuscript; available in PMC: 2024 Jun 13.

Published in final edited form as: Pharm Res. 2014 Sep 4;32(3):863–875. doi: 10.1007/s11095-014-1502-4

Figure 3. — a. CEM cells were treated with different concentrations of curcumin (Cur), DMC and 5AC for 72 h followed by DNA extraction, DNA bisulfite treatment and DNA pyrosequencing of seven CpG sites in the CpG island of the p15 gene as described under methods. Data represent the average of duplicates ± SD and * indicates statistically significant difference from the control at p<0.05. b. CEM cells were treated with different concentrations of curcumin (Cur), DMC and 5AC for 72 h followed by DNA extraction, DNA bisulfite treatment and DNA pyrosequencing of five CpG sites in the CpG island of the CDH-1 gene as described under methods. Data represent the average of duplicates ± SD and * indicates statistically significant difference from the control at p<0.05. c. CEM cells were treated with high concentrations (5 and 10 uM) of curcumin (Cur) and 5AC (1 uM) for 72 h followed by DNA extraction, DNA bisulfite treatment and DNA pyrosequencing of seven CpG sites in the CpG island of the p15 gene and five CpG sites in the CpG island of the CDH-1 gene as described under methods. Data represent the average of duplicates ± SD and * indicates statistically significant difference from the control at p<0.05.

Figure 3. — a. CEM cells were treated with different concentrations of curcumin (Cur), DMC and 5AC for 72 h followed by DNA extraction, DNA bisulfite treatment and DNA pyrosequencing of seven CpG sites in the CpG island of the p15 gene as described under methods. Data represent the average of duplicates ± SD and * indicates statistically significant difference from the control at p<0.05. b. CEM cells were treated with different concentrations of curcumin (Cur), DMC and 5AC for 72 h followed by DNA extraction, DNA bisulfite treatment and DNA pyrosequencing of five CpG sites in the CpG island of the CDH-1 gene as described under methods. Data represent the average of duplicates ± SD and * indicates statistically significant difference from the control at p<0.05. c. CEM cells were treated with high concentrations (5 and 10 uM) of curcumin (Cur) and 5AC (1 uM) for 72 h followed by DNA extraction, DNA bisulfite treatment and DNA pyrosequencing of seven CpG sites in the CpG island of the p15 gene and five CpG sites in the CpG island of the CDH-1 gene as described under methods. Data represent the average of duplicates ± SD and * indicates statistically significant difference from the control at p<0.05.