FIG. 1.

Schematic structures of test vectors to examine transcription regulatory functions of three different MARs of HPV-16 (A) and deletions of the E6-MAR (B). The recombinant reporter gene (uppermost scheme) consisted of a contiguous genomic segment of the HPV-16 LCR with the epithelial cell-specific enhancer (E) and the E6 promoter P97 (P) fused to the luciferase gene (Luc). This reporter gene was linked in natural or artificial sequence to the MARs in the 5′ part of the LCR (L), in the E6 gene (E6), or overlapping with the E5 gene and the early-late intergenic region (E5). Details of the constructs are described in Materials and Methods.