FIG. 2.

MARs of HPV-16 in the 5′ LCR, the E6 gene, and the E5 gene repress transcription from the HPV-16 enhancer-promoter in transient transfections of HeLa cells. The presence of the MAR in the 5′ part of the LCR in its natural position relative to the EP segments (L-EP-Luc) represses luciferase reporter gene expression by 68%, while various artificial constructs reduce enhancer-promoter activity by 60% (L-EP-Luc-L) to 87% (L-EP-Luc-E5). Schematic structures of the test vectors are shown in Fig. 1.