FIG. 8.

Mutational analysis of one of the CDP/Cut binding sites within the E6 MAR of HPV-16. CDP/Cut does not bind to a particular highly conserved sequence but rather binds to AT-rich sequences that normally include the sequence 5′-TAAT-3′ (3, 28). This behavior is confirmed by an EMSA analysis of mutations of the CDP/Cut binding site within E6-10. Alteration of the TAAT element in the 3′ part of this oligonucleotide either eliminates (V and VI) or reduces (II and III) CDP/Cut binding, while alteration of two other AT-rich stretches (I and IV) has no effect on the CDP/Cut band shift.