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Total ionic current (TIC) of recombinant Mpro incubated with BPE and sequentially digested with trypsin and chymotrypsin. Above is shown the Mpro sequence with the peptide portions identified by nLC-HR-MS/MS analysis highlighted in green. Symbols above the sequence indicate modifications detected as follows: C, carbamidomethylation; O, oxidation; * delphinidin-3-glucoside/galactoside Micheal adduct on His163 or His164.
